>
产品中心 >
Cell_transfection >
Signagen/AAV-CMV-FLPo (AAV Serotype 1)/SL101455/>30 µL (Std Pack)
ProductCategory: Pre-maderecombinantAAV;AAV-CMV-FLPo;AAV-FLPo;AAV1-CMV-FLPo;AAV1-FLPo
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheointegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV1-CMV-FLPopre-packagedrAAVinSEROtype1(withcapsidfromAAVserotype1and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderCMVpromoter. Readytouseformat.
Titer: >1x10^13 VG/mL
Size: > 30 µL(StdPack)
StorageBuffer: SterilePBS
InfectionProtocol: 
VectorMap: 
